GSEA and expression analysis revealed that NGFR+ tumors indeed expressed high levels of MRD-associated genes (Fig. 4c, right panel and Supplementary Fig. 5d, left panel), particularly the MRD-associated gene EHD3 was upregulated in post-treatment melanoma and was significantly more highly expressed in NGFRhigh MBM (Fig. 4d, left panel). This evidence concerns the gene EHD3 and melanoma.