We examined the levels of Ecad and NGFR in a lymph node metastasis (LN-MET) and concordant MBM (M1, M4) and validated a co-occurrence of melanoma cells that featured distinct (~35% NGFR+; ~50% Ecad+) and overlapping (~10% NGFR+/Ecad+) phenotypes (Fig. 4b, left panels and Supplementary Fig. 5c). This evidence concerns the gene NGFR and melanoma.