The initial TargetSeq provided information on the mutation status of hot spot regions of 50 cancer-associated genes and revealed that MBM (n = 29) either harbored mutations in BRAF or NRAS with variant allele frequencies (VAF) of 0.91–0.26 (BRAFV600) or 0.87–0.40 (NRASQ61/G13), in line with previous observations3,61. Here, BRAF is linked to cancer.