PFKP and glioblastoma: To determine whether PFKP expression is required for the angiogenesis-mediated continued growth of GBM tumors, we depleted PFKP expression using PFKP short hairpin RNA (shRNA) in LN229 human GBM cells overexpressing constitutively active EGFRvIII mutant (LN229/EGFRvIII) (Supplementary Fig. S1A), which lacks 267 amino acids from its extracellular domain and is frequently found in GBM [13], and intracranially injected the cells into mice.