Growth of brain tumors (Fig. 4A), HIF-1α expression, β-catenin S552 phosphorylation, VEGF expression, and blood vessel formation (Fig. 4B) in mice implanted with PFKP-depleted LN229/EGFRvIII cells expressing rPFKP Y64F were decreased compared with those in mice implanted with the cells expressing its WT counterpart, and this reduction was restored by expression of an active AKT1 mutant (Fig. 4A, B). The gene discussed is VEGFA; the disease is brain neoplasm.