DIAPH3 and Familial prostate cancer: Kim et al. demonstrated that heparin-binding EGF activates EGFR and ERK1/2 signaling to induce shedding of exosome-size EVs, and that loss of Diaphanous-related formin-3, a cytoskeletal regulator, also promotes the release of EVs in amoeboid prostate cancer cells [141].