Since DAZL−/− glioma cells were not viable in culture possibly attributed to a crucial role of DAZL or germ cell fate in immortality of tumour cells, U251- DAZL+/−, LN229-DAZL+/− and A172mut- DAZL+/− cells were studied (Additional file 1: Table S2). This evidence concerns the gene DAZL and neoplasm.