In order to test the hypothesis that CXCL1 promotes colorectal cancer progression, we used CRISPR/Cas9 technology to knockout CXCL1 in MC38 colon cancer cells to construct MC38CXCL1−/− cell line; in addition, we also used “Sleeping Beauty” transposon to transduce CXCL1 into MC38 cells to construct MC38CXCL1+/+ cell line. The gene discussed is CXCL1; the disease is colorectal cancer.