Later, we analyzed the expression of CXCR2 in various groups of mouse tumors by immunohistochemistry, and the results showed that CXCR2 was localized on the colon cancer cell membrane and cytoplasm (Fig. 7d), which provided the possibility of binding to CXCL1, but the protein expression level of CXCR2 was negligible compared to the high level of CXCL1 expression in colon cancer cell lines. Here, CXCR2 is linked to malignant colon neoplasm.