At 12 weeks of HFD challenge, the mice lacking GPSM1 exclusively in myeloid lineage cells were partially resistant to obesity, exhibiting much lower body weights and fat mass without significant changes in their lean mass (Fig. 2a, b and Supplementary Fig. 3a, b), which were demonstrated by dramatic decreases in fat-pad weight, including scWAT, perirenal WAT (periWAT), and brown adipose tissue (BAT) (Fig. 2c and Supplementary Fig. 3c). This evidence concerns the gene GPSM1 and obesity due to melanocortin 4 receptor deficiency.