Hypergeometric distribution analysis showed that TM4SF1 + cancer cells (Epi-C0) and UBE2C + cancer cells (Epi-C6) were enriched in the cancer region, and CAFs (Fib-1) and Tregs (T/NK-C6: FOXP3) colocalized with the above cancer cell subclusters in the cancer region (Fig. 6d). The gene discussed is FOXP3; the disease is cancer.