As expected, TM4SF1 + cancer cells (Epi-C0), 2 subtypes of NK cells (T/NK-C4: GNLY and T/NK-C5: NKG7) and mast cells (Mye-C0: TPSB2) were colocalized in the cancer region, while Tregs (T/NK-C6: FOXP3) were not found in the cancer region (Fig. 6d). Here, TM4SF1 is linked to cancer.