PROX1 and liver cancer: To further confirm these results in the Prox1 liver-specific knockout mice, we used a DEN-induced liver cancer mouse model and observed that the dietary intake of high BCAA promoted liver tumourigenesis in the Prox1f/f mice and abolished the tumour incidence caused by Alb-Cre; Prox1f/f mice, as the tumour number and liver-body ratio did not yield a difference between Prox1f/f and Alb-Cre; Prox1f/f mice following BCAA treatment (Fig. 6a–c).