SPP1 and hepatocellular carcinoma: We found significantly decreased levels of Afp (a marker of undifferentiated HCC), Spp1, Gpc3 (markers of early HCC), and Epcam (a marker of stemness; Fig. 3E, H), suggesting that Myc-R26Met tumours might be more differentiated and at more advanced stages than Alb-R26Met tumours.