To demonstrate that SIRT1 mRNA upregulation has epigenetic effects in older 3xTg-AD mice after Nosustrophine treatment, we immunoblotted for a chromatin-associated substrate of SIRT1, acetylated-histone H3 (Lys14), in mice hippocampi (Figure 10A,B). The gene discussed is SIRT1; the disease is Alzheimer disease.