In our study, we found that SA significantly downregulated the protein levels of TLR-4, NF-κB, NLRP3, caspase-1 and IL-1β in the livers of NAFLD rats, indicating the inhibition of the TLR-4/NF-κB/NLRP3 pathway. This evidence concerns the gene NLRP3 and metabolic dysfunction-associated steatotic liver disease.