Our data displayed that the protein expression levels of inflammation-related genes, such as TLR-4, NF-κB, NLRP3, caspase-1 and IL-1β, were elevated in NAFLD rats, indicating the activation of the TLR-4/NF-κB/NLRP3 inflammatory pathway. This evidence concerns the gene NLRP3 and metabolic dysfunction-associated steatotic liver disease.