TNF and necrotizing enterocolitis: Inflammation biomarkers, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κβ), tumor necrosis factor-alpha (TNF-α), interleukin one beta (IL-1β), and interleukin 6 (IL-6) were all analyzed via RT-qPCR (Figure 2) due to the use of these biomarkers in other NEC models and clinical trials [108,109,110,111,112].