Moreover, Zhao et al. reported that an 8-week 14:10 TRF (eating until 19:30) in 15 males with obesity restored circadian rhythms in glucoregulatory hormones and led to increased CLOCK and decreased PER2 expression in subcutaneous adipose tissue (SAT) at midnight [86]. This evidence concerns the gene CLOCK and obesity due to melanocortin 4 receptor deficiency.