CLOCK and obesity due to melanocortin 4 receptor deficiency: However, in a short-term study of 11 males with overweight/obesity, the 16:8 TRF (eating between 10:00–18:00) compared to the extended feeding (eating between 07:00–22:00) was found to alter the rhythmicity of serum and muscle metabolites and regulate the rhythmicity of genes controlling amino acid transport, without aggravating core clock gene expression [85].