Fumagalli et al. found that the accumulation of genetic mutations in the Wnt, epidermal growth factor receptor (EGFR), P53 and TGF-β signaling pathways can drive CRC cells to migrate and grow at distant sites in an orthotopic organoid transplantation model and in engineered human colon tumor organoids [65]. This evidence concerns the gene EGFR and colorectal carcinoma.