MLH1 and colorectal cancer: Thus, a combination treatment regimen of 5-fluorouracil and curcumin in two colorectal cancer cell lines (HCT116 and HCT116+ch3, which was made MLH1-proficient by the stable transfection of chromosome 3 bearing the hMLH1 gene) induced the expression or cleavage of pro-apoptotic proteins (caspase-8, -9, and -3; PARP; and Bax) and downregulated anti-apoptotic (BCL-XL) and proliferative (cyclin D1) proteins.