The above results are consistent with previous research on human esophageal cancer mutations [22,25,26,27,28,29], and we found that these genes (Lrp1b, Trp53, Notch1, Pcdh9, Syne1, Xirp2, Csmd3, and Muc4) showed mutations at high frequencies in our mice model. Here, TP53 is linked to esophageal cancer.