The above results are consistent with previous research on human esophageal cancer mutations [22,25,26,27,28,29], and we found that these genes (Lrp1b, Trp53, Notch1, Pcdh9, Syne1, Xirp2, Csmd3, and Muc4) showed mutations at high frequencies in our mice model. Here, NOTCH1 is linked to esophageal cancer.