In agreement with previous evidence [28], our study showed that infection of primary bEECs with E. coli significantly increased the expression of IL-1β and IL-18 and that the NLRP3 inflammasome simultaneously was assembled, which manifested as NLRP3 activation and cleavage of Caspase-1 into its p22 active form. The gene discussed is NLRP3; the disease is infection.