Luminex and CBA quantification confirmed high levels of CHI3L1 (0–6705 pg/mL), IGFBP2 (309.5–3454 pg/mL), Serpin E1 (328.4–6833 pg/mL), Angiogenin (27.27–614.3 pg/mL), IL-6 (12.57–788.1 pg/mL), CXCL8 (IL-8) (359.9–1317 pg/mL), CCL2 (MCP-1) (522.1–7786 pg/mL), and VEGF (0–5370 pg/mL) in all serum-derived glioblastoma primary lines (Figure 4). This evidence concerns the gene CHI3L1 and glioblastoma.