RACGAP1 and colonic neoplasm: Figure 2A–G demonstrate that the expression of the RACGAP1 protein was significantly elevated in the colon, HNSC, hepatocellular carcinoma, LUAD, PAAD, UCEC, and colon cancer compared to normal tissues (p < 0.001). Subsequently, we retrieved the IHC figures for the normal and malignant tissues to validate our earlier results. Normal tissues of the colon, nasopharynx, liver, lung, ovary, pancreas, and endometrium did not exhibit any staining, so the findings were consistent. Similarly, it was moderate to high in the comparable malignant samples (Figure 2A–E).