Next, the presence of SapS-Spot was determined in the supernatants (by immunoprecipitation with anti-Spot magnetic beads; IP) and cytosol-, membrane-, and cell wall-fractions of S. aureus cell suspensions grown for 4 h in TSB (Figure 2A), or in lysed macrophage supernatants and intracellular persistent bacteria at 3 and 5 h after infection, respectively (Figure 2B). The gene discussed is SKAP2; the disease is infection.