In vivo, LCn3-PUFA decreased ACE2 in tissues of obese fa/fa Zucker rats (Figure 1e), which have endothelial dysfunction [53], whereas in vitro, this reduction was only observed in the growing, activated state of EA.hy926 cells (Figure 2e), which likewise represented endothelial dysfunction [26]. This evidence concerns the gene ACE2 and endothelial dysfunction.