To assess whether the cell-free-produced RIT was able to specifically kill cells carrying its target receptor CD7, we performed an MTT assay with NTC, scFv, PE24, and RIT on Raji (CD7−), Jurkat, HSB-2, and ALL-SIL (CD7+) cell lines (Figure 5 and Figure S7). This evidence concerns the gene CD7 and acute lymphoblastic leukemia.