TNF and metabolic dysfunction-associated steatotic liver disease: In this study, mice with NAFLD presented negative effects on key proteins in TJ integrity by reducing Occludin concentration and expression, and increased Tnf-α expression inducing inflammation, in accordance with a study with obese mice [37,38], while an improvement of the abnormal distribution of Occludin and Zo-1 and Cdh-1 expression and a reduced expression of inflammatory-related genes was induced by MI treatment in NAFLD mice.