Studies in animals with diet-induced obesity showed significant changes in the hippocampus and frontal cortex, including microglial activation, the dysfunction of transient receptor potential (TRP) ion channels, or lower expression of choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT) [53,54]. This evidence concerns the gene CHAT and obesity due to melanocortin 4 receptor deficiency.