In order to test the effect of CDT intoxication under more in vivo conditions, we first analyzed the alterations of Arp2 distribution (by using a monoclonal anti-Arp2 antibody) and the actin and tubulin cytoskeleton of human colon carcinoma Caco2 cells after intracellular translocation of recombinant CDTa by CDTb (Figure 5A–C). Here, ACTR2 is linked to colon carcinoma.