To dissect novel estrogen-dependent lncRNAs in breast cancer cells, we performed RNA-sequencing (RNA-seq) in ER-positive MCF7 cells treated with 17β-estradiol (E2, 100 nM), E2 (100 nM) with OH-tamoxifen (OHT, 1 μM), or a control vehicle for 4 h. Here, ESR1 is linked to breast cancer.