We found that wild-type TP53 expression in H2172 reduced the numbers of LC3 puncta and suppressed the conversion of cytosolic LC3 (LC3-I) to the lipidated form of LC3 (LC3-II), as revealed by the LC3 conversion assay with or without the treatment of BafA1, suggesting that TP53 inhibits the early stage of autophagy in lung cancer cells (Figure 5B–D). The gene discussed is TP53; the disease is lung cancer.