Since that IFN-γ derived from activated CD8+ T-cells in TME can trigger tumor cell ferroptosis upon anti-PD-1 antibody treatment, and targeting Wnt/β-catenin signaling can exacerbate ferroptosis according to our results, we proposed that targeting Wnt/β-catenin might increase the effectiveness of anti-PD-1 antibody by the induction of tumor cell ferroptosis. The gene discussed is CD8A; the disease is neoplasm.