Figure 4B demonstrates that both B16-conditioned medium and TGF-β activate SC to produce and release similar levels of PGE2. To verify that B16-derived TGF-β is responsible for PGE2 upregulation in SC, B16-conditioned medium was generated in the presence of neutralizing anti-TGF-β antibody. Figure 4C shows that neutralization of TGF-β significantly attenuated the ability of tumor cells to stimulate SC for PGE2 production. Here, TGFB1 is linked to neoplasm.