To understand the underlying mechanisms by which the RPE cells could induce microglia activation during AMD pathogenesis, we performed scRNAseq analysis on cells isolated from the SRS (including RPE and choroid) of aged (15 months old) Cryba1fl/fl and Cryba1 cKO mice [23] and identified 35 different cell clusters including the RPE [23] along with immune cell (microglia, neutrophils, monocytes) populations (Supplementary Figure S2), that have been previously documented to be important in AMD pathogenesis [10,11,12,13,14,15,16,17,18]. The gene discussed is CRYBA1; the disease is age-related macular degeneration.