To assess the spatiotemporal localization of the activated microglia in aged Cryba1 cKO mice, we performed immunofluorescence studies on retinal cryo-sections and found that microglia (Iba1-positive cells) home into the SRS of aged (9 months old; the age when the AMD-like phenotype is observed in this mouse model) Cryba1 cKO retina, but not in age-matched floxed (Cryba1fl/fl) controls (Figure 1a). This evidence concerns the gene AIF1 and age-related macular degeneration.