High ACY1 expression was associated with lymphatic invasion and perineural invasion in READ in TCGA datasets (Figure 2D), lymph node metastases in CRC in the GSE4459 dataset (Figure 2E), and the American Joint Committee on Cancer stage (AJCC) for COAD in the GSE38832 dataset (Figure 2F). This evidence concerns the gene ACY1 and colorectal carcinoma.