To test whether ulixertinib can inhibit cell proliferation and promote apoptosis in human NB cells, we first treated a panel of NB cell lines and PDX cell lines, including five MYCN-amplified (LAN-1, IMR-32, NGP, SK-N-BE(2), and CHLA136), four MYCN non-amplified (CHLA255, SH-SY5Y, SK-N-AS, and LAN-6), four NB PDX MYCN-amplified (COG-N-519x), and MYCN non-amplified (COG-N-564x, COG-N-618x, and COG-N-700x) cell lines. The gene discussed is MYCN; the disease is neuroblastoma.