Normal and tumour tissues from such patients were analysed by qRT-PCR for the following 12 genes; six from RNA-seq: CLDN1, MAGEB2, CD24, CEACAM6, IL1B and ISG15 and six from RNA-seq and proteomics cross-linking: AKR1C3, TNFAIP2, RAB7A, LGALS3BP, PSCA and SSRP1. The gene discussed is ISG15; the disease is neoplasm.