In one study investigating the effect of IL-33 on Tregs, the recombinant human IL-33 was added to co-cultures of human-laryngeal-squamous-cell-carcinoma-cell-line-stimulated dendritic cells and healthy donor CD4+ T cells, and the IL-10 and TGF-β levels were elevated, whereas ST2 blockade diminished the production of IL-10 and TGF-β [61], consistent with what we found in this study. The gene discussed is TGFB1; the disease is laryngeal squamous cell carcinoma.