Notably, the S100A7 abundance in subclinical mastitis samples had little difference compared with healthy samples (Figure 1D, p > 0.05) while there was a significant decrease in clinical mastitis samples (Figure 1D, p < 0.01), and there was also a significant difference between subclinical and clinical mastitis samples (Figure 1D, p < 0.05). The gene discussed is S100A7; the disease is mastitis.