2) We explored many novel NAFLD-associated marker genes in these hepatic immune cells, including lower Thbs1 and Serpina1a expression; higher Lgals3, Ctss, Cd48, and B2m as NAFLD general markers and Fcgr2b, Spp1, Cxcl2 and Elane as aggravation markers in both humans and mice. This evidence concerns the gene ELANE and metabolic dysfunction-associated steatotic liver disease.