2) We explored many novel NAFLD-associated marker genes in these hepatic immune cells, including lower Thbs1 and Serpina1a expression; higher Lgals3, Ctss, Cd48, and B2m as NAFLD general markers and Fcgr2b, Spp1, Cxcl2 and Elane as aggravation markers in both humans and mice. The gene discussed is THBS1; the disease is metabolic dysfunction-associated steatotic liver disease.