The qRT–PCR results were basically consistent with the scRNA-seq analysis (Figure 5A), especially Tspo, Lgals3, Slpi and Serpina1a. In particular, Ccr2, which could promote obesity-associated macrophage infiltration in hepatic tissue to induce inflammation and angiogenesis (33), showed no obvious expression differences between WT and KO mice by scRNA-seq analysis, but its expression changed significantly after enlarging the samples (using qRT–PCR). The gene discussed is TSPO; the disease is obesity due to melanocortin 4 receptor deficiency.