In this study, the macrophage subsets 0-Mφ-Thbs1, 3-Mφ-Gngt2 and 4-Mφ-Cxcl2 were MDMs that make up the bulk of macrophages in NAFLD, while 2-Mφ-Cxcl9 was an MTRs population. This evidence concerns the gene CXCL9 and metabolic dysfunction-associated steatotic liver disease.