In our study, 187 patients with NSCLC and 310 age- and gender-matched controls were included, as well as an independent set of 29 patients for validation. Dual specificity phosphatase 6 (DUSP6), EIF2S3 eukaryotic translation initiation factor 2 subunit gamma (eukaryotic translation initiation factor 2 subunit gamma), growth factor receptor bound protein 2 (GRB2), MDM2 proto-oncogene (MDM2), neurofibromin 1 (NF1), POLDIP2, ring finger protein 4 (RNF4), and WEE1 were identified as significant NSC (WEE1 G2 checkpoint kinase). This evidence concerns the gene RNF4 and non-small cell lung carcinoma.