POLDIP3-(POLDIP3 transcript lacking exon 3 and 29 amino acids) expression was significantly increased in liver cancer tissues compared to paired adjacent normal liver tissues. POLDIP3-overexpression significantly increased the proliferation and migration of HCC cells and promoted the growth of xenotransplantation in in vitro and in vivo functional experiments. POLDIP3-will be a potential target for the treatment of liver cancer. This evidence concerns the gene POLDIP3 and liver cancer.