Western blot was performed to validate the differentially translated genes (DTGs): Ndufa13 with decreased translation efficiency in LF showed reduced protein level in LF (Additional file 1: Fig. S5a), Gng11 with increased translation efficiency in LF showed increased protein level in LF (Additional file 1: Fig. S5b), and Ptx3 with increased translation efficiency in HF showed increased protein level in HF (Additional file 1: Fig. S5c). The gene discussed is GNG11; the disease is hydrops fetalis.