To capture the extent of intratumor heterogeneity and to identify treatments that can enhance sensitivity to ALK inhibition in ALK‐rearranged lung cancer, we characterized tumor tissues (n = 6) and cultures (n = 4) established from multiple tumor regions (TRs) of a large surgically resected tumor (9 × 12 × 9 cm) from a 55‐year‐old never‐smoker female patient with ALK‐rearranged T3N2M0 stage lung adenocarcinoma with diffuse metastasis. Here, ALK is linked to lung cancer.