Since it is well-known that the NCCR is a hypervariable region that may determine viral replication efficiency, and cellular tropism and NCCR rearrangements have been associated with specific human diseases’ development, such as nephropathy for BKPyV and PML for JCPyV [17,27], in this study, we characterized, in positive samples, HPyVs NCCR sequences. The gene discussed is PML; the disease is Nephropathy.