The studies utilizing biochemical CsA-washout assay, which is based on the ablation of restriction to the HIV-1 infection imposed by TRIM-Cyp by the addition of CsA and the determination of the percentage of HIV-1 infected cells that become insensitive to TRIM-Cyp (relative half-life of uncoating), also support the model that CA is gradually lost from the capsid core with a half-life of less than 1 h in the cytosol. The gene discussed is TRAT1; the disease is HIV-1 infection.