TP53 and cancer: We had already found above that the TME immune cell infiltration of NEC cluster2 was remarkably increased; thus, the highly activated angiogenesis, as well as the EMT pathway, also proved our hypothesis that NEC cluster2 was a typical immune-excluded phenotype, and that NEC cluster3 markedly presented pathway enrichment associated with pathways in cancers, including the transforming growth factor (TGF)-β signaling pathway, WNT signaling pathway and P53 signaling pathways.