Finally, through a combination of bioinformatics analysis of public datasets and mass spectrometry assay analysis of clinical tissue samples, we identified differential expression of ANGPTL6, TTC36, SLC27A5, ADH4, PHYHD1, MBL2, GYS2, CYP2C9, CLEC4M, and CLEC4G between HCC tissues and adjacent nontumorous tissues at the transcriptional and protein levels. Here, SLC27A5 is linked to hepatocellular carcinoma.