Somatic mutation analysis revealed that the top 10 genes (TP53, APC, FBXW7, ABCA2, ACADL, UCP1, VASH2, ZNF488, TACC3, and TREML2) were rearranged in the site of two primary malignancies that participated in chromosomal instability (CIS), Wnt signaling pathway (WNT), negative regulation of lipid biosynthetic process (LBP), mitochondrial inner membrane (MiM), microtubule-binding (MB), gliogenesis (Gilo), spindle (Spindle), and T-cell activation (TAct) (Figure 5B, Figure S1). Here, TREML2 is linked to in situ carcinoma.