Clusters 1, 3, 4,5 are hardly present in the naïve dataset, but are highly induced upon infection, with two clusters (4 and 5) annotated as monocytes expressing high levels of monocyte-specific genes like Ly6c2, Plac8, and Itgam (CD11b) and low levels of macrophage-specific genes like Adgre1 (F4/80) or C1qc. The other two clusters (1 and 3) were annotated as monocytes-derived-macrophages (MDM) 1 and 2, respectively, since they express high levels of macrophages markers, while maintaining constant level of monocyte markers. The gene discussed is ADGRE1; the disease is infection.