To validate the differentiation pattern specific to deep invasion of EVTs, we compared the invasive PAS placenta from the second trimester (15 and 23 weeks) with the gestational age-matched placenta, using immunohistochemistry staining with LAMB4 and KRT6A. These controls had previous CS history, with the location of all placentas covered the anterior wall (Supplementary Table S1). The gene discussed is LAMB4; the disease is Cowden syndrome 1.