The results of western blot analysis illustrated that TROAP silence could significantly downregulate the level of downstream target genes of PI3K/Akt/GSK-3β signaling, such as PI3K, p-AKT, and p-GSK3B in HCC-LM3 and SW620 cells compared to control (p < 0.05, Figures 4G, H). Here, TROAP is linked to hepatocellular carcinoma.