These results, therefore, reinforce our previous knowledge on the dysregulation of the spliceosomal landscape in cancer cells10, 25, 26 and provide a solid evidence of novel and relevant splicing‐related elements (i.e. EIF4A3) that are consistently altered and exert an important role in HCC, which further strengthen the relationship and implication of the splicing process and the development and progression of HCC. Here, EIF4A3 is linked to hepatocellular carcinoma.