Moreover, the in vitro modulation of EIF4A3 expression with specific siRNAs or its pharmacological inhibition decreased key functional parameters of aggressiveness, including proliferation, migration, invasion, tumoursphere size and colony formation in all liver cancer cell lines analysed and reduced tumour growth in a preclinical model (Hep3B‐induced xenograft tumours). Here, EIF4A3 is linked to liver cancer.