In particular, considering that tumor cells are most likely exposed to cytokines produced by tumor infiltrating lymphocytes, we studied the effects of the triple blockade of MEK, PD-L1 and Ido-1 on patients’ derived three dimensional samples, using tumor spheroids from NSCLC patient's biopsy, generated as previously described [6]. The gene discussed is MAP2K7; the disease is non-small cell lung carcinoma.