To further explore the function of UBR7 on HCC tumorigenesis, we generated liver-specific UBR7 knockout mice (Alb-Cre; UBR7fl/fl mice) by crossing UBR7fl/fl mice with albumin-Cre mice, and UBR7 silencing in the liver was confirmed by immunoblotting and IHC staining (Supplementary Fig. 3A, 3B). The gene discussed is UBR7; the disease is hepatocellular carcinoma.